Evaluation of Calcium Concentration in Solution - in Vitro Study of Fluoride Action on Dental Enamel

In the present study the mineral loss/gain (∆Ca) in dental enamel during de-/ remineralization process, depending on used fluoride concentration, were measured in vitro in pH cycling conditions. 20 sound premolars extracted for orthodontic reasons were divided in 4 subgroups. Artificial lesions were created on each of them. They were all subject to a pH cycling regimen for 2 weeks. During the pH cycling regimen each of the 4 subgroups received different treatment: Placebo paste, fluoride tooth paste (1440 ppm F), fluoride gel (12,000 ppm F), and rinsing solution (227 ppm F). Calcium concentration in de-/remineralization solution at the beginning and at the end of the study was determined using the spectrometer with atomic absorption. The relative succession of the fluoride containing products in terms of efficiency was established to be: fluoride tooth paste ≥ fluoride rinse > fluoride gel.